Jumping Spider’s Eyes
The head of a jumping spider is prepared with fluorescent markers and imaged using a confocal laser scanning microscope. Both images actually depict the same side and depth level of the specimen’s head; the photomicrograph on the left has been flipped horizontally for a side-by-side comparison of fluorescence.
To fit a particular experiment, specific fluorescent dyes are often selected after careful consideration. In both of these images, cell nuclei are visualized with TO-PRO®-3, a monomeric cyanine stain with far-red fluorescence. On the left, autofluorescence is visualized in blue; on the right, microfilaments (cyan) are visualized with rhodamine phalloidin.
Images are by Igor Siwanowicz, Max Planck Institute of Neurobiology, Munich, Germany.