A longitudinal cross-section of the Arabidopsis root meristem with “Brother of Brainbow” clones is imaged with a Leica SP2 confocal microscope. The middle panel shows a root tip 3 days after induction of CRE recombinase, leading to the formation of broad clones. The outer roots show the six possible expression combinations as a result of single (CFP or RFP together with YFP), double (magenta, CFP, or RFP), or no (YFP) recombination events in the parental germ cells, mediated by CRE.
By Guy Wachsman, Renze Heidstra, Ben J. Scheres, Utrecht University, Netherlands
© 2011 by the American Society of Plant Biologists